Thermo Fisher Scientific Fluorescein Polyclonal Antibody, DyLight 549
DyLight 549 형광 표지를 가진 Fluorescein Polyclonal Antibody로, FITC 라벨 단백질 검출에 적합. Western blot, ICC/IF, ELISA 등 다양한 형광 응용 가능. 염소 유래 IgG 폴리클로날 항체로 높은 특이성과 안정성 제공. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:25,000 |
| Immunocytochemistry (ICC/IF) | >1:5,000 |
| ELISA | >1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Fluorescein conjugated to Goat IgG |
| Conjugate | DyLight™ 549 |
| Excitation / Emission Max | 556 / 570 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C and avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Fluorescein conjugated Bovine Serum Albumin.
Target Information
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light, exciting molecules to emit visible yellow-green light. This emission stops when the excitation light is removed.
Fluorochrome labeling enables rapid and accurate localization of antigen-antibody interactions in cells or tissues.
FITC is a commonly used marker for immunofluorescent techniques because conjugation to proteins is simple and retains biological activity.
Anti-FITC antibodies can isolate FITC-labeled cells using magnetic bead sorting or reduce background signals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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