Thermo Fisher Scientific Biotin Polyclonal Antibody, DyLight 549
DyLight 549 형광체로 표지된 Biotin 폴리클로날 항체로 Western blot, ICC/IF, ELISA에 사용 가능. 염소 유래 IgG이며 높은 특이성과 감도를 제공. 연구용으로 안정적이며 장기 보관 시 -20°C에서 유지.
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Thermo Fisher Scientific Biotin Polyclonal Antibody, DyLight 549
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | >1:10,000 |
| Immunocytochemistry (ICC/IF) | >1:5,000 |
| ELISA | >1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host/Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH) |
| Conjugate | DyLight™ 549 |
| Excitation/Emission Max | 556/570 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | 0.02M potassium phosphate, pH 7.2, with 10mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Biotin conjugated IgG, and Biotin conjugated Bovine Serum Albumin.
Target Information
Biotin is a water-soluble B complex vitamin (Vitamin B7).
It is required by all organisms and can only be synthesized by bacteria, yeasts, molds, algae, and some plant species.
Biotin is essential for incorporation of carbon dioxide into organic compounds and exhibits a molecular weight of approximately 244 Da.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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