Thermo Fisher Scientific GFP Polyclonal Antibody, DyLight 549

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Store vial at 4°C prior to restoration.
• For extended storage, aliquot contents and freeze at -20°C or below.
• Avoid cycles of freezing and thawing.
• Centrifuge product if not completely clear after standing at room temperature.
• Product is stable for several weeks at 4°C as an undiluted liquid.
• Dilute only prior to immediate use.

Assay Information:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria).
No reaction was observed against Human, Mouse, or Rat serum proteins.

Target Information

The jellyfish Aequorea victoria contains Green Fluorescent Protein (GFP Tag) that emits light in its bioluminescence reaction. GFP is widely used as a reporter protein for gene expression in both eukaryotic and prokaryotic systems and as a protein tag in cell culture and multicellular organisms.
As a fusion tag, GFP enables the localization and tracking of proteins and facilitates research into subcellular dynamics. GFP is a 27 kDa monomeric protein that autocatalytically forms a fluorescent pigment. The wild-type protein absorbs blue light (max 395 nm) and emits green light (peak 508 nm) without requiring cofactors.
GFP fluorescence is stable, species-independent, and suitable for multiple applications including gene expression monitoring, protein localization, protein-protein interaction studies (yeast two-hybrid system), and FRET-based distance measurements.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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