Thermo Fisher Scientific CD90 (Thy-1) Monoclonal Antibody (eBio5E10 (5E10)), Brilliant Violet 480, eBioscience
인간 CD90(Thy-1) 항원을 인식하는 eBio5E10 단클론 항체로, Brilliant Violet 480 형광체로 결합되어 있습니다. 유세포 분석(Flow Cytometry)에 적합하며, 405 nm 레이저 장비에서 사용 가능합니다. 장기 배양 가능한 세포군 분석에 유용합니다.
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Thermo Fisher Scientific CD90 (Thy-1) Monoclonal Antibody (eBio5E10 (5E10)), Brilliant Violet 480, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.125 µg)/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Brilliant Violet™ 480, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio5E10 (5E10) |
| Conjugate | Brilliant Violet™ 480 |
| Excitation / Emission Max | 437 / 478 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2925633 |
Additional Formats
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395
- Brilliant UV 563
- Brilliant Violet 650
- Brilliant Violet 786
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine7
- PerCP-Cyanine5.5
- PerCP-eFluor 710
- Super Bright 436
- Request custom conjugation
Product Specific Information
The eBio5E10 monoclonal antibody reacts with human CD90, also known as Thy-1 (thymus cell antigen-1). CD90 is a 25–35 kD receptor expressed on thymocytes, CD34+ prothymocytes, hematopoietic stem cells, neurons, and subsets of fetal liver, cord blood, and bone marrow cells.
This antibody is suitable for flow cytometric analysis and has been tested using HEL cells at 5 µL (0.125 µg) per test.
Brilliant Violet™ 480 (BV480) emits at 479 nm and is intended for use with violet (405 nm) lasers. Ensure instrument compatibility before use.
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.
BV480 is not compatible with methanol-based fixation.
Excitation: 440 nm; Emission: 479 nm; Laser: Violet Laser.
BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.
Target Information
CD90 (Thy-1) antigen is a GPI-linked glycoprotein in the Immunoglobulin superfamily. It is expressed in murine T cells, thymocytes, neural cells, Kupffer’s cells, and fibroblasts.
CD90 plays a role in cell-cell or cell-ligand interactions during synaptogenesis and other neural events. It serves as a marker for stem cells and axonal processes of mature neurons.
Structural studies of CD90 contributed to the foundation of the Immunoglobulin superfamily and the first biochemical characterization of a vertebrate GPI anchor.
Diseases associated with CD90 dysfunction include nasopharyngeal carcinoma and thymoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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