Thermo Fisher Scientific CD62L (L-Selectin) Monoclonal Antibody (MEL-14), Brilliant Violet 480, eBioscience

Applications

Flow Cytometry (Flow)

• Tested Dilution: 0.125 µg/test

Product Specifications

Additional Formats Available

• Alexa Fluor 561
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 496 / 615 / 737 / 805
• Brilliant Violet 421 / 605 / 650 / 786
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 510 / 555 / 585 / 610-30S / 660-40S
• NovaFluor Red 710
• NovaFluor Yellow 590 / 660
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436 / 600 / 702 / 780
• Request custom conjugation

Product Specific Information

The MEL-14 monoclonal antibody reacts with mouse CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells. It binds glycosylated, fucosylated, and sialylated glycoproteins such as CD34, glycam-1, and MAdCam-1, mediating lymphocyte rolling and homing to high endothelial venules (HEV) of peripheral lymphoid tissues.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes. Recommended use ≤0.125 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize titration for best performance.

Fluorochrome Information:
Brilliant Violet™ 480 (BV480) emits at 479 nm and is excited by a 405 nm violet laser. Ensure instrument compatibility.

Staining Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.

Fixation Compatibility:
Brilliant Violet™ 480 (BV480) is not compatible with methanol-based fixation.

Excitation/Emission: 440 nm / 479 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

Human CD62L (L-Selectin) is a 74–95 kDa glycoprotein adhesion receptor composed of:

• C-type lectin binding domain
• EGF-like domain
• Two short consensus repeat (SCR) sequences
• Spacer region, transmembrane region, and cytoplasmic tail

It is constitutively expressed on most leukocytes, including lymphocytes (excluding many memory T cells), monocytes, and polymorphonuclear cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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