Thermo Fisher Scientific CD69 Monoclonal Antibody (FN50), Brilliant Violet 480, eBioscience

Thermo Fisher Scientific CD69 Monoclonal Antibody (FN50), Brilliant Violet 480, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.125 µg)/test
Publications: -

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 561
• Alexa Fluor 660
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395
• Brilliant UV 615
• Brilliant UV 661
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 650
• Brilliant Violet 711
• Brilliant Violet 786
• eFluor 450
• FITC
• NovaFluor Blue 610-70S
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 780
• Request custom conjugation

Product Specific Information

The FN50 monoclonal antibody reacts with human CD69, also known as very early activation antigen (VEA). CD69 (~30 kDa) is expressed on the cell surface as a disulfide-linked dimer and is rapidly upregulated upon activation, found on lymphocytes, monocytes, and platelets.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
  • Use at 5 µL (0.125 µg) per test
  • Test defined as staining a cell sample in 100 µL final volume
  • Applicable cell count range: 10⁵–10⁸ cells/test

Fluorophore Information:
Brilliant Violet™ 480 (BV480) emits at 479 nm and is intended for use with a violet (405 nm) laser. Ensure instrument compatibility.

Buffer Recommendation:
When using multiple polymer dye-conjugated antibodies (e.g., Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (#SB-4401-42) or Brilliant Stain Buffer™ (#00-4409-75) to minimize non-specific interactions.

Fixation Compatibility:
BV480 is not compatible with methanol-based fixation.

Spectral Properties:
Excitation: 440 nm; Emission: 479 nm; Laser: Violet Laser.

Trademark Notice:
BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen.™

Target Information

CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide-bonded homodimer (~60 kDa under non-reducing conditions). It contains one or two N-linked oligosaccharides and is present on activated platelets. CD69 expression is induced upon activation of T lymphocytes and may play a role in proliferation and signal transduction in lymphocytes, NK cells, and platelets. Alternative splicing results in multiple transcript variants. Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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