
Thermo Fisher Scientific CXCL12 alpha (SDF-1 alpha) Polyclonal Antibody, PeproTech
Human CXCL12 alpha (SDF-1 alpha)에 특이적인 Rabbit Polyclonal Antibody로, Western blot, ELISA 등 다양한 응용에 적합. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 민감도를 제공. 연구용으로만 사용 가능.
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Thermo Fisher Scientific CXCL12 alpha (SDF-1 alpha) Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | View 4 publications |
| Immunocytochemistry (ICC/IF) | – | View 1 publication |
| Flow Cytometry (Flow) | – | View 1 publication |
| ELISA | 0.5–2.0 µg/mL | View 3 publications |
| Neutralization (Neu) | – | View 2 publications |
| In vitro Assay (IV) | – | View 2 publications |
| Miscellaneous PubMed (Misc) | – | View 1 publication |
| Immunostaining (IS) | – | View 7 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse, Rat |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human SDF-1alpha (CXCL12) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | −20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930821 |
Product Specific Information
AA Sequence of recombinant protein:
KPVSLSYRCPCRFFESHVARANVKHLKILNTPNCALQIVARLKNNNRQVCIDPKLKWIQEYLEKALNKPreparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human SDF-1alpha (CXCL12). The antibody was purified by affinity chromatography employing an immobilized Human SDF-1alpha (CXCL12) matrix.Sandwich ELISA:
For detection of Human SDF-1alpha (CXCL12) by sandwich ELISA, use 0.5–2.0 µg/mL antibody (100 µL/well). In conjunction with PeproTech Biotinylated Anti-Human SDF-1alpha (CXCL12) (500-P87ABt) as detection antibody, it detects ≥0.2–0.4 ng/well of Recombinant Human SDF-1alpha.Western Blot:
For detection of Human SDF-1alpha by Western Blot, use 0.1–0.2 µg/mL antibody. Detection limit for Recombinant Human SDF-1alpha is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Target Information
CXCL12 is a stromal cell-derived alpha chemokine member of the intercrine family. Together with its receptor CXCR4, it activates lymphocytes and is implicated in cancer metastasis such as breast cancer. Mutations in CXCL12 are associated with resistance to HIV-1 infection. Multiple transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일명: 500-P87A-100UG_CXCL12_alpha_P48061-1_Rabbit.svg, 500-P87A-100UG_CXCL12_alpha_P48061-1_Rabbit_PDP.jpeg)
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