Thermo Fisher Scientific H3K27me3 Chimeric Recombinant Rabbit Monoclonal Antibody (BT164)
H3K27me3 표지를 인식하는 재조합 토끼 단클론 항체로, 인간 시료에 반응합니다. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 사용 가능합니다. HEK293 세포에서 발현되었으며, Protein A로 정제된 고순도 항체입니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 0.0003–0.01 µg/mL | |
| Immunohistochemistry (IHC) | Assay-dependent | |
| Immunocytochemistry (ICC/IF) | 10 µg/mL | |
| Flow Cytometry (Flow) | 1:100 | |
| ELISA | Assay-dependent | |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BT164 |
| Immunogen | BT164 is a mice-derived lupus antibody established by selection on apoptotic chromatin. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3074756 |
Product Specific Information
Specificity: The epitope of BT164 is mapped in the N-terminal tail of histone H3 (27-KSAPAT-32) and includes the apoptosis-induced trimethylation of K27.
Target Information
H3K27me3 is the epigenetic mark where histone H3 is trimethylated at lysine residue 27. It serves as a silencing mark associated with downregulation of nearby genes and is typically found in heterochromatin regions and inactive gene promoters. H3K27me3 often opposes the activating mark H3K4me3 and is closely linked to developmental gene regulation.
EZH2, a component of the PRC2 complex, is the histone methyltransferase responsible for trimethylating H3K27, while UTX and JMJD3 act as demethylases for H3K27me3. Dysregulation of this modification is implicated in various diseases, including cancer. H3K27me3 antibodies are commonly used in western blot and ChIP assays to study epigenetic regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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