Thermo Fisher Scientific H4ac pan-acetyl (K8,K12,K16) Chimeric Recombinant Rabbit Monoclonal Antibody (KM-2)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (IHC)

Assay-dependent

-

Immunocytochemistry (ICC/IF)

10 µg/mL

-

Flow Cytometry (Flow)

1:100

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG, kappa

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

KM-2

Immunogen

KM-2 antibody was derived from an MRL/lpr lupus mouse.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.02% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3074755

Product Specific Information

Specificity: KM-2 antibody recognizes the N-terminal peptides 1-20 of histone H2A and 1-29 of histone H4 which have a large sequence homology. Histone H4 acetylation on lysines 8, 12 and 16 significantly increases the antibody`s binding while the acetylation at K5 decreases it.

Target Information

H4 acetylation at lysine residues 8, 12, and 16 is a well-characterized post-translational modification that is associated with transcriptional activation. This modification is catalyzed by histone acetyltransferases (HATs) and is often found at promoter regions of actively transcribed genes. It is believed to facilitate chromatin remodeling and transcription factor recruitment, ultimately leading to increased gene expression. However, the precise mechanisms by which H4 acetylation at these specific lysine residues contributes to gene regulation are still under investigation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.