Thermo Fisher Scientific Occludin Monoclonal Antibody (OC-3F10)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-1.0 µg/mL

View 138 publications 138 publications

Immunohistochemistry (IHC)

-

View 56 publications 56 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 9 publications 9 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 21 publications 21 publications

Immunocytochemistry (ICC/IF)

2-3 µg/mL

View 124 publications 124 publications

Flow Cytometry (Flow)

-

View 2 publications 2 publications

ELISA (ELISA)

0.1-1.0 µg/mL

View 2 publications 2 publications

Immunoprecipitation (IP)

-

View 2 publications 2 publications

in situ PLA (PLA)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 41 publications 41 publications

Product Specifications

Species Reactivity

Dog, Human, Mouse, Rat

Published species

Bovine, Cynomolgus monkey, Dog, Guinea pig, Human, Mouse, Pig, Rat, Rhesus monkey, Sheep

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

OC-3F10

Immunogen

GST fusion protein consisting of the C-terminal region (~150aa) of human occludin. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Occludin,uniProtId:Q16625-1,ncbiNodeId:9606,antigenRange:150,antigenLength:522,antigenImageFileName:33-1500_Occludin_Q16625-1_House_mouse.svg,antigenImageFileNamePDP:33-1500_Occludin_Q16625-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Alexa Fluor 488
• Alexa Fluor 594
• FITC
• HRP
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533101

Target Information

Occludin is a 65 kDa protein that can exist in a variety of phosphorylated forms, ranging up to approximately 82 kDa. Occludin is thought to be involved in regulating both the localization and the function of occludin. Polyunsaturated fatty acids are known to up-regulate occludin expression, increasing the transendothelial cell resistance and reducing the cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly and continuously expressed at cell-cell contact sites, whereas non-neural tissues show lower expression and discontinuous distribution. Overall structural features of the occludin protein are highly conserved in all the species examined. Under-expression of tight junction proteins, including occludin, is a key molecular abnormality responsible for the increased permeability of tumor endothelial tight junctions, which contributes to brain tumor edemas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.