Thermo Fisher Scientific CD62P (P-Selectin) Monoclonal Antibody (AK-4), FITC, eBioscience
인간 CD62P(P-Selectin)을 인식하는 AK-4 단클론 항체로, FITC 형광 표지되어 유세포분석(Flow Cytometry)에 적합합니다. 활성화된 혈소판 및 내피세포 표면의 P-Selectin 검출에 사용되며, 488 nm 레이저에서 520 nm 파장으로 방출됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | 5 µL (1 µg)/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | AK-4 |
| Conjugate | FITC |
| Excitation/Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10668715 |
Available Formats:
Product Specific Information
Description:
The AK-4 monoclonal antibody reacts with human CD62P (P-Selectin), a 140 kDa transmembrane glycoprotein. Upon activation, CD62P is rapidly transported from its cytoplasmic stores in the Weibel-Palade bodies of endothelial cells or the alpha-granules of platelets to the cell surface. CD62P mediates adhesion between endothelial cells and neutrophils or monocytes during inflammation and also facilitates platelet-leukocyte interactions.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Pre-titrated and tested by flow cytometric analysis of resting and thrombin-activated human platelets. Recommended usage is 5 µL (1 µg) per test.
A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL.
Cell number should be empirically determined (10⁵–10⁸ cells/test).
Fluorescence Characteristics:
- Excitation: 488 nm
- Emission: 520 nm
- Laser: Blue Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
P-selectin (CD62P) is a 140 kDa type I transmembrane glycoprotein involved in cell adhesion processes. It is stored in Weibel-Palade bodies of endothelial cells and α-granules of platelets and is rapidly translocated to the cell surface upon activation by thrombin, histamine, complement 5a, Ca²⁺ ionophores, or ADP.
P-selectin mediates interactions between activated endothelial cells or platelets and leukocytes, playing a key role in inflammation, thrombosis, and atherogenesis. It binds to sialylated Lewis blood group carbohydrate antigens on neutrophils and monocytes and is used clinically to distinguish heparin-induced thrombocytopenia with or without thrombosis.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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