
Thermo Fisher Scientific CD62L (L-Selectin) Monoclonal Antibody (SK11), FITC, eBioscience
인간 및 비인간 영장류 CD62L(L-Selectin)을 인식하는 SK11 단클론 항체로, FITC 형광 표지되어 유세포분석(Flow Cytometry)에 최적화됨. 5 µL/test로 사용하며, 488 nm/520 nm에서 검출 가능. PBS/BSA 완충액에 보관, 4°C 암소 저장.
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Thermo Fisher Scientific CD62L (L-Selectin) Monoclonal Antibody (SK11), FITC, eBioscience™
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.125 µg)/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Baboon, Chimpanzee, Cynomolgus monkey, Human, Non-human primate, Rhesus monkey |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SK11 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10597901 |
Product Specific Information
This SK11 monoclonal antibody reacts with human and non-human primate CD62L (also known as L-selectin and LECAM).
CD62L is expressed on neutrophils, monocytes, and subsets of T, B, and NK cells, as well as endothelial cells.
It binds glycosylated, fucosylated, and sulfated sialylated glycoproteins such as CD34, glycam-1, and MadCAM-1, mediating lymphocyte rolling and homing to high endothelial venules (HEV) of peripheral lymphoid tissues.
The SK11 antibody recognizes chimpanzee, rhesus, cynomolgus monkey, olive baboon, and pigtailed macaque as well as pig.
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells.
Use at 5 µL (0.125 µg) per test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test.
Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Human CD62L is a 74–95 kDa glycoprotein of the selectin family of adhesion receptors.
It includes:
- Aminoterminal C-type lectin binding domain
- EGF-like domain
- Two SCR sequences homologous to complement binding proteins
- Short spacer, transmembrane, and cytoplasmic regions
CD62L is constitutively expressed on leukocytes including lymphocytes (except memory T cells), monocytes, and polymorphonuclear cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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