Thermo Fisher Scientific CD65 Monoclonal Antibody (VIM8), FITC, eBioscience
CD65 항원을 인식하는 VIM8 단클론 항체로, FITC 형광 표지되어 유세포분석(Flow Cytometry)에 최적화됨. 인간 세포 반응성, 마우스 IgM 아이소타입. AML 연구 및 골수세포 발현 분석에 유용. 4°C 보관, 동결 금지.
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Applications
Flow Cytometry (Flow)
- Tested Dilution: 5 µL (0.25 µg)/test
- Applications Reported: Flow cytometric analysis of human peripheral blood cells
- Applications Tested: Pre-titrated and validated for use at 5 µL (0.25 µg) per test
- Recommended Laser: Blue (Excitation 488 nm; Emission 520 nm)
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgM |
| Recommended Isotype Control | Mouse IgM Isotype Control (11E10), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | VIM8 |
| Conjugate | FITC |
| Excitation/Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS (pH 7.2) with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10853177 |
Product Specific Information
This VIM8 antibody reacts with the desialylated asialoganglioside of human CD65.
CD65 is a type 2 fucosylated carbohydrate antigen expressed on all myeloid cells during development. It is highly expressed on granulocytes and weakly on monocytes in peripheral blood. CD65 acts as a ligand for CD62E (E-Selectin).
The sialylated form, CD65s, serves as a marker for acute myeloid leukemia (AML), with expression increasing as CD34 decreases. CD65 is also important for extravascular infiltration of AML cells.
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD65 is a fucosylated carbohydrate antigen found on developing myeloid cells.
It is highly expressed on granulocytes and weakly on monocytes.
CD65 interacts with CD62E (E-Selectin) and its sialylated form (CD65s) is a useful marker for AML progression.
It plays a key role in the extravascular infiltration of AML cells.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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