Thermo Fisher Scientific SOX2 Monoclonal Antibody (Btjce), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

0.2-1 µg/mL

View 4 publications 4 publications

Immunohistochemistry (IHC)

-

View 31 publications 31 publications

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

1-5 µg/mL

View 29 publications 29 publications

Flow Cytometry (Flow)

Assay-Dependent

View 5 publications 5 publications

ELISA (ELISA)

Assay-Dependent

-

Product Specifications

Species Reactivity

Human, Mouse

Published species

Bovine, Chicken, Human, Mouse, Non-human primate

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

Btjce

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Alexa Fluor 488
• eFluor 570
• eFluor 660
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2865465

Product Specific Information

Description: The Btjce monoclonal antibody reacts with the transcription factor Sox2, a member of the SOX (sex determining region Y -related HMG (High Mobility Group) Box) family of proteins. Sox family members play a role in early organ development, and in particular, Sox2 is essential for regulating genes that control normal mammalian embryogenesis. Sox2 and family member Sox3 are expressed as early as the preimplantation and epiblast stages respectively. Later expression is restricted to the neuroepithelium. Sox2 has been shown to be necessary for maintaining self-renewal and pluripotency of mouse and human embryonic stem (ES) cells (ESC). Oct4 (POU5F1), Klf4, c-myc, and Sox2 were the original four factors used to reprogram differentiated mouse and human cells to induced pluripotent stem cells (iPSC).

Expression of Sox2 is tightly regulated and recent studies have demonstrated that small changes in the levels of Sox2 in ES cells can trigger differentiation into multiple cell types. Sox2 expression is not limited to ES cells, it is also essential for early neurogenesis where its expression becomes restricted to the neural plate, and later to neural stem cells where it functions to suppress neural differentiation. Sox2 in combination with other stem cell markers can be used to characterize stem cell populations. Ectopic expression of Sox2 has been associated with multiple cancer types including colorectal and breast.

Applications Reported: This Btjce antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, immunocytochemistry, western blot and ELISA (Fluorochrome-conjugated Btjce is recommended for use in intracellular flow cytometry).

Applications Tested: This Btjce antibody has been tested by immunohistochemistry on FFPE human testes at less than or equal to 5 µg/mL. The antibody can be used with either high or low pH antigen retrieval. This Btjce antibody has been tested by immunocytochemistry on fixed and permeabilized iPS cells at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Bacterial expression of region after aa135 (homology between species but not to other family members)

Target Information

SOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of the SOX2 gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. The SOX2 gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Further, SOX2 protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation, optic nerve hypoplasia and syndromic microphthalmia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.