Thermo Fisher Scientific TRMT12 Monoclonal Antibody (OTI3D1), TrueMAB
TRMT12 단백질을 표적으로 하는 Mouse IgG2b 단일클론 항체로, Western blot에 최적화되어 있습니다. 인간 TRMT12 재조합 단백질로 면역화되었으며, 무보존제 PBS 버퍼에 동결건조 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific TRMT12 Monoclonal Antibody (OTI3D1), TrueMAB
Applications
- Western Blot (WB)
Tested Dilution: 1:500–1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3D1 |
| Immunogen | Full length human recombinant protein of human TRMT12 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
TYW2, also known as TRM12, is an enzyme involved in the wybutosine-tRNA (Phe) biosynthesis pathway. Wybutosine (yW) is a hypermodified guanosine at the 3′ position adjacent to the anticodon of phenylalanine tRNA, stabilizing codon–anticodon interactions during ribosomal decoding.
TYW2 participates in a multistep enzymatic process that ensures accurate translation and has been found amplified or overexpressed in various breast cancer cell lines and tumor samples, suggesting a potential role in breast cancer development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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