Thermo Fisher Scientific IFN gamma Monoclonal Antibody (XMG1.2), Alexa Fluor 561, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Intracellular Staining)
• Tested Dilution: ≤ 0.06 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 660
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 615 / 661 / 737 / 805
• Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
• eFluor 450 / 660
• FITC
• Functional Grade
• PE / PE-Cyanine7 / PE-eFluor 610
• PerCP-Cyanine5.5
• Request custom conjugation

Product Specific Information

The XMG1.2 antibody reacts with mouse interferon (IFN) gamma, a 20 kDa cytokine produced by activated T, B, and NK cells with antiviral and antiparasitic activity.
This antibody is neutralizing and suitable for intracellular staining followed by flow cytometric analysis.

• Alexa Fluor 561 may cause non-specific staining in Swiss Webster and SJL mice, but not in BALB/c or C57BL/6 strains.
• Tested using stimulated mouse splenocytes with the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00).
• Recommended usage: ≤ 0.06 µg per test in 100 µL final volume.
• Excitation: 558 nm / Emission: 575 nm (Yellow-Green Laser)
• Designed for use on spectral cytometers, multiplexable with PE and PE-eFluor 610.

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor primarily produced by T-lymphocytes and NK cells in response to antigens and cytokines.
It functions as a homodimer (~45 kDa) glycoprotein and shows species-specific activity (no cross-reactivity between human and mouse IFNγ).

Key functions include:

• Antiviral and antiproliferative activity
• Induction of MHC class I and II expression
• Macrophage activation
• Enhanced immunoglobulin secretion by B cells

IFNγ acts via IFNγ receptor I/II and activates the JAK-STAT pathway. Expression is upregulated by IL-2, FGF basic, EGF, and downregulated by vitamin D3 or DMN.
Mutations in the IFNγ gene are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.