Thermo Fisher Scientific CYP8B1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 6 publications 6 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to amino acids 416-461 of human CYP8B1 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CYP8B1,uniProtId:Q9UNU6-1,ncbiNodeId:9606,antigenRange:416-461,antigenLength:501,antigenImageFileName:PA5-37088_CYP8B1_Q9UNU6-1_Rabbit.svg,antigenImageFileNamePDP:PA5-37088_CYP8B1_Q9UNU6-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2553905

Product Specific Information

This antibody detects endogenous protein at a molecular weight of 58 kDa.

Purity is >95% by SDS-PAGE.

Target Information

CYP8B1 (sterol 12-alpha-hydroxylase) is a member of the cytochrome P450 superfamily of monooxygenase enzymes that are involved in the metabolism of a wide array of endogenous and xenobiotic compounds. CYP8B1 is highly expressed in liver and is an important enzyme for bile acid synthesis. Specifically, CYP8B1 moderates the ratio of cholic acid over chenodeoxycholic acid to control the solubility of cholesterol. The gene encoding human CYP8B1 maps to chromosome 3p22.1. The CYP8B1 gene encodes a 501-amino acid protein and does not contain any introns. The CYP8B1 gene promoter is transactivated by hepatocyte nuclear factor 4a. In mice, disruption of the CYP8B1 gene prevents the synthesis of cholate, which is a primary bile acid.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.