Thermo Fisher Scientific NSE Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes followed by cooling at room temperature for 20 minutes. After antigen retrieval, incubate samples with primary antibody for 30 minutes at room temperature. A suggested positive control is pancreas tissue.

Target Information

Neuron specific enolase (NSE, also known as ENO1, ENO2, ENO3) is an enzyme involved in glycolysis and gluconeogenesis, catalyzing the conversion of 2-phosphoglycerate to phosphoenolpyruvate. NSE is highly stable in biological fluids and can diffuse into cerebrospinal fluid (CSF) when neuronal membranes are damaged.

NSE is one of three mammalian enolases:

• ENO1 (α-enolase): expressed in most tissues
• ENO2 (γ-enolase / NSE): expressed primarily in neurons and neuroendocrine cells
• ENO3 (β-enolase): expressed in muscle

Co-expression of NSE and chromogranin A is common in neuroendocrine neoplasms. Antibodies to NSE are useful for identifying neuronal cell bodies, developing neuronal lineages, and neuroendocrine cells. NSE release into CSF and blood serves as a biomarker of neuronal injury and is clinically used as a marker in neurological disorders such as stroke, hypoxic brain damage, status epilepticus, Creutzfeldt-Jakob disease, and herpetic encephalitis. Elevated NSE levels are also found in certain neoplasias, including pediatric neuroblastoma and small cell lung cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.