Thermo Fisher Scientific OCT1 (POU2F1) Polyclonal Antibody
Rabbit polyclonal antibody targeting human OCT1 (POU2F1), suitable for WB, IHC(P), and GS. Predicted to react with multiple species. Supplied in liquid form, unconjugated, and ideal for transcription factor studies. For research use only.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Gel Shift (GS) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide derived from the C-terminus of human Oct-1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2549991 |
Product Specific Information
This antibody is predicted to react with chicken, amphibian, mouse, and rat based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes followed by cooling at room temperature for 20 minutes.
Following antigen retrieval, incubate samples with primary antibody for 30 minutes at room temperature.
A suggested positive control is tonsil tissue.
Target Information
OCT1 (POU2F1) is a ubiquitously expressed transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates promoters of genes for small nuclear RNAs (snRNA), histone H2B, and immunoglobulins.
It modulates transcription transactivation by NR3C1, AR, and PGR.
During human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with viral transactivator protein VP16 and HCFC1, enabling transcription of viral immediate early genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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