Thermo Fisher Scientific PI3K p85/p55 Recombinant Rabbit Monoclonal Antibody (10L6B7)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:4,500-1:18,000

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

10L6B7

Immunogen

A synthetic peptide of human PI3 Kinase p85 alpha/beta (NP_852664.1). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PI3K p85/p55,uniProtId:P27986-1,ncbiNodeId:9606,antigenRange:465-469,antigenLength:724,antigenImageFileName:MA5-51324_PI3K_p85p55_P27986-1_Rabbit.svg,antigenImageFileNamePDP:MA5-51324_PI3K_p85p55_P27986-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.59 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_3094402

Product Specific Information

Immunogen sequence: RLYEE

Target Information

Phosphoinositide 3-kinase (PI3K, phospho PI3K) Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Phospho PI3K is necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. During insulin stimulation, it also binds to IRS-1. Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.