Thermo Fisher Scientific AF9 Recombinant Rabbit Monoclonal Antibody (1T4V6)
Recombinant rabbit monoclonal antibody against human AF9 (MLLT3). Validated for WB and ELISA. Expressed in HEK293 cells with high purity via affinity chromatography. Supplied as liquid, 1 mg/mL in PBS with BSA and glycerol. For research use only.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 1T4V6 |
| Immunogen | Recombinant fusion protein containing amino acids 1–150 of human MLLT3/AF9 (NP_004520.2) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 0.05% BSA, 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_3093118 |
Product Specific Information
Immunogen sequence:
MASSCAVQVK LELGHRAQVR KKPTVEGFTH DWMVFVRGPE HSNIQHFVEK VVFHLHESFP RPKRVCKDPP YKVEESGYAG FILPIEVYFK NKEEPRKVRF DYDLFLHLEG HPPVNHLRCE KLTFNNPTED FRRKLLKAGG DPNRSIHTSS
Target Information
The human AF9 (MLLT3) gene is a common fusion partner with the ALL1 (MLL) gene at 11q23, resulting in the translocation t(9;11)(p22;q23). The AF9 gene spans over 100 kb and includes two breakpoint cluster regions (BCRs):
- BCR1 within intron 4 (site A)
- BCR2 spanning introns 7 and 8 (site B)
AF9 contains structural elements such as a DNA topoisomerase II cleavage site and DNase I hypersensitive site within intron 7 (BCR2). Two scaffold-associated regions (SAR1 in intron 4 and SAR2 encompassing exons 5–7) flank these cleavage sites. These regions share structural features with the MLL breakpoint cluster region, supporting a model of DNA breakage and repair–mediated recombination between MLL and AF9.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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