Thermo Fisher Scientific TEV Cleavage Site Monoclonal Antibody (9A10-4C3)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

-

Product Specifications

Species Reactivity

Tag

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

9A10-4C3

Immunogen

KLH conjugate peptide representing the cleavage site recognized by TEV enzyme

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536832

Product Specific Information

MA1-124 has been successfully used in Western blot applications.

The MA1-124 immunogen is a peptide representing the cleavage site recognized by TEV enzyme.

Target Information

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tandem affinity purification (TAP) is an affinity purification method for isolation of TAP-tagged proteins together with associated proteins. The protocol involves the fusion of the TAP tag (typically a calmodulin binding peptide (CBP), a tobacco etch virus protease (TEV protease) cleavage site and Protein A) to the protein of interest. The TAP technique is useful in analyzing in vivo interactions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.