Thermo Fisher Scientific p130 Monoclonal Antibody (KAB40)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

KAB40

Immunogen

GST fusion protein to the N terminus of p130.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Protein A/G

Storage buffer

PBS

Contains

0.08% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1077383

Product Specific Information

This antibody recognizes both the phosphorylated or unphosphorylated forms of p130 at a MW of 130kDa. It is suitable for use in Western blot with His-tagged or GST-tagged proteins.

Target Information

RBL2 (also known as p130) or Retinoblastoma-like protein 2 is a key regulator of entry into cell division. It is directly involved in heterochromatin formation by maintaining overall chromatin structure and (in particular) that of constitutive heterochromatin by stabilizing histone methylation. RBL2 recruits and targets histone methyltransferases KMT5B and KMT5C leading to epigenetic transcriptional repression. It controls histone H4 Lys20 trimethylation. It probably also acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. RBL2 serves as a potent inhibitor of E2F-mediated trans-activation and associates preferentially with E2F5.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.