Thermo Fisher Scientific LEF1 Monoclonal Antibody (REMB6)
LEF1 단백질을 인식하는 마우스 단클론 항체로, Western blot, IHC, ICC, ChIP 등 다양한 응용에 사용 가능. 인간 및 생쥐 시료에 반응하며, Jurkat 세포 용해물이 양성 대조군으로 권장됨. Wnt 신호전달 관련 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–5 µg/mL | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 | - |
| ChIP assay (ChIP) | 2.5 µg/10⁶ cells | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | REMB6 |
| Immunogen | Full-length recombinant LEF-1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | PBS |
| Contains | 0.08% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2234575 |
Product Specific Information
A suggested positive control for this product is Jurkat cell lysate.
Target Information
This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The encoded protein binds to a functionally important site in the T-cell receptor-alpha enhancer, conferring maximal enhancer activity. It is involved in the Wnt signaling pathway and may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been identified in sebaceous tumors and linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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