Thermo Fisher Scientific Hexanoyl-Lysine adduct Monoclonal Antibody (5D9), PerCP
Hexanoyl-Lysine adduct(HEL) 변형 단백질을 특이적으로 검출하는 PerCP 결합 단일클론 항체. Western blot, IHC, ICC, Flow cytometry, ELISA에 적합. 보존제 무첨가, 4°C 보관. 단백질 산화 스트레스 바이오마커 연구에 활용 가능.
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Thermo Fisher Scientific Hexanoyl-Lysine adduct Monoclonal Antibody (5D9), PerCP
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:100 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:25–1:100 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| Flow Cytometry (Flow) | 1:100 |
| ELISA | 1:1,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5D9 |
| Immunogen | Synthetic Hexanoyl modified Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | PerCP |
| Excitation / Emission Max | 482 / 675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932233 |
Additional Formats Available
- Unconjugated ([MA5-27554])
- APC ([MA5-45777])
- FITC ([MA5-45778])
- PE ([MA5-45780])
- Request custom conjugation (Thermo Fisher form)
Product Specific Information
A 1:1,000 dilution of MA5-45779 was sufficient for detection of Hexanoyl-Lysine adduct in 0.5 µg of Hexanoyl-Lysine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for Hexanoyl-Lysine adduct (HEL) modified peptides and proteins. Does not detect free Hexanoyl-Lysine.
Does not cross-react with Acrolein, Crotonaldehyde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.
Target Information
Hexanoyl-Lysine (HEL) is a novel lipid peroxidation biomarker derived from the oxidation of omega-6 unsaturated fatty acids.
HEL may be used as a biomarker in omega-6 PUFA (polyunsaturated fatty acid) oxidation in living organisms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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