Thermo Fisher Scientific Hexanoyl-Lysine adduct Monoclonal Antibody (5D9), FITC
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FITC 형광 표지된 Hexanoyl-Lysine adduct 단일클론 항체로, HEL 변형 단백질 검출에 특이적입니다. Western blot, IHC, ICC, Flow cytometry, ELISA 등 다양한 응용에 사용 가능합니다. 단백질 산화 스트레스 및 지질 과산화 바이오마커 연구에 적합합니다.
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Thermo Fisher Scientific Hexanoyl-Lysine adduct Monoclonal Antibody (5D9), FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:100 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:25–1:100 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| Flow Cytometry (Flow) | 1:100 |
| ELISA | 1:1,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Chemical |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5D9 |
| Immunogen | Synthetic Hexanoyl modified Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | FITC |
| Excitation/Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932232 |
Available Formats
- Unconjugated (MA5-27554)
- APC (MA5-45777)
- PE (MA5-45780)
- PerCP (MA5-45779)
- Custom conjugation available upon request
Product Specific Information
- A 1:1,000 dilution of MA5-45778 detects Hexanoyl Lysine adduct in 0.5 µg of Hexanoyl Lysine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as secondary antibody.
- Specific for Hexanoyl-Lysine adduct (HEL) modified peptides and proteins.
- Does not detect free Hexanoyl-Lysine.
- No cross-reactivity with Acrolein, Crotonaldehyde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.
Target Information
Hexanoyl-lysine (HEL) is a novel lipid peroxidation biomarker derived from the oxidation of omega-6 unsaturated fatty acids. HEL may serve as a biomarker in omega-6 PUFA (polyunsaturated fatty acid) oxidation studies in living organisms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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