Thermo Fisher Scientific Rat anti-Mouse IgG2a Secondary Antibody, Biotin, eBioscience

Applications

Tested Dilution

Immunohistochemistry (Paraffin) (IHC (P))

10 µg/mL

Immunocytochemistry (ICC/IF)

10 µg/mL

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rat / IgG1, kappa

Class

Monoclonal

Type

Secondary Antibody

Clone

m2a-15F8

Conjugate

Biotin Biotin Biotin

View additional formats

• [APC ](/antibody/secondary/product-name/Mouse IgG2a/conjugates/APC/host/Rat/)
• [eFluor 570](/antibody/secondary/product-name/Mouse IgG2a/conjugates/eFluor 570/host/Rat/)
• [FITC](/antibody/secondary/product-name/Mouse IgG2a/conjugates/FITC/host/Rat/)
• [HRP](/antibody/secondary/product-name/Mouse IgG2a/conjugates/HRP/host/Rat/)
• [PE ](/antibody/secondary/product-name/Mouse IgG2a/conjugates/PE/host/Rat/)
• [PE-Cyanine7](/antibody/secondary/product-name/Mouse IgG2a/conjugates/PE-Cyanine7/host/Rat/)
• [PerCP-eFluor 710](/antibody/secondary/product-name/Mouse IgG2a/conjugates/PerCP-eFluor 710/host/Rat/)
• [Super Bright 436](/antibody/secondary/product-name/Mouse IgG2a/conjugates/Super Bright 436/host/Rat/)
• [Super Bright 600](/antibody/secondary/product-name/Mouse IgG2a/conjugates/Super Bright 600/host/Rat/)
• [Super Bright 780](/antibody/secondary/product-name/Mouse IgG2a/conjugates/Super Bright 780/host/Rat/)
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2572785

Target

IgG2a

Antibody Form

Whole Antibody

Product Specific Information

Description: The monoclonal antibody m2a-15F8 recognizes Mouse IgG2a antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG2a or any rat isotype antibodies.

Applications Reported: This m2a-15F8 antibody has been reported for use in immunohistochemical staining and immunocytochemistry.

Applications Tested: This m2a-15F8 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue and by immunocytochemistry on fixed MDCK cells. The antibody can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.