Thermo Fisher Scientific APC Monoclonal Antibody (eBioAPC-6A2), Biotin, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.015 µg/test

-

Product Specifications

Host/Isotype

Mouse / IgG1

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioAPC-6A2

Conjugate

Biotin Biotin Biotin

View additional formats

• Unconjugated
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_657507

Product Specific Information

Description: The eBioAPC-6A2 monoclonal antibody reacts with allophycocyanin (APC) and APC-Cy7, with no crossreactivity to fluorescein (FITC) or phycoerythrin (PE). When using eBioAPC-6A2 as a second-step antibody, in combination with an appropriate third step, to bind a APC-conjugated primary antibody, there is no decrease in the fluorescent intensity of the directly conjugated APC antibody. Therefore no interference or quenching of the APC fluorochrome is observed. The eBioAPC-6A2 can be used for separation of cells labeled with APC-conjugated antibodies or for staining.

Please note that the optimal concentration for this antibody will vary depending on the density of the primary APC-conjugated antibody and the application tested.

Applications Reported: This eBioAPC-6A2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioAPC-6A2 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells stained with an APC-conjugated antibody. This can be used at less than or equal to 0.015 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Anti-APC (Allophycocyanin) antibodies can be used to bind APC-conjugated primary antibodies without decreasing fluorescent intensity of the APC fluorochrome. This method is used for separation of cells labeled with APC-conjugated antibodies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.