Thermo Fisher Scientific CTNNA3 Recombinant Rabbit Monoclonal Antibody (JE55-32)
CTNNA3 단백질을 인식하는 재조합 토끼 단클론 항체로, 인간·마우스·랫트 시료에 반응합니다. Western blot 및 IHC(P) 검증 완료. 단백질 A로 정제된 액상 항체이며, 세포골격 내 알파카테닌 단백질 연구에 적합합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JE55-32 |
| Immunogen | Recombinant fragment within C-terminal Human CTNNA3 (UniProt ID: Q9UI47-1, antigen length: 895 aa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2911629 |
Product Specific Information
- Positive Control: Rat heart tissue lysate, mouse heart tissue lysate, 293 cell lysate, mouse heart tissue, rat heart tissue
- Subcellular Location: Cytoskeleton
Target Information
Catenins are proteins that connect cadherin/beta-catenin cell adhesion complexes to the actin cytoskeleton. Although originally discovered in testis, alphaT-catenin is expressed in other tissues, with the highest levels observed in heart. Immunohistochemical analysis showed human alphaT-catenin localization at intercalated discs of cardiomyocytes and in peritubular myoid cells of testis. It has been demonstrated that alphaT-catenin can restore cell-cell adhesion in colon cancer cells lacking alpha-catenins, indicating its role in forming specific cell-cell contacts in muscle cells. The encoded protein has a predicted size of approximately 100 kDa and shares about 57% amino acid identity with other alpha-catenins. In cells transfected with alphaT-catenin cDNA, interaction with alpha-catenin was confirmed by coimmunoprecipitation. The 892_24D2S antibody against alphaT-catenin does not cross-react with alphaE-catenin or alphaN-catenin in western blot analysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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