Thermo Fisher Scientific CD71/Transferrin Receptor (TFRC) (Extracellular Domain) Monoclonal Antibody (TFRC, 1839)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Peptide Array (Array)

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

• For IHC on PFA-fixed tissues, incubate antibody for 30 minutes at room temperature.
• Staining of formalin-fixed tissues requires heating sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release, and the apotransferrin-receptor complex is recycled to the cell surface. The transferrin receptor is essential for erythrocyte and nervous system development. Serum transferrin receptor (sTfR) is used to detect erythropoietin (EPO) misuse and as a diagnostic marker for various anemias, including those related to rheumatoid arthritis, pregnancy, irritable bowel syndrome, and HIV.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.