Thermo Fisher Scientific PTX3 Monoclonal Antibody (2C3)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

-

Immunocytochemistry (ICC/IF)

Assay-Dependent

-

Flow Cytometry (Flow)

Assay-Dependent

-

ELISA (ELISA)

Assay-Dependent

-

Immunoprecipitation (IP)

Assay-Dependent

-

Functional Assay (Functional)

Assay-Dependent

-

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2C3

Immunogen

Recombinant mouse PTX3

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

100 µg/mL

Purification

Protein G

Storage buffer

PBS with 0.1% BSA

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2815514

Product Specific Information

Product is supplied at 1 mL (100 µL/mL).

Application Notes: Western blot- A non-reduced sample treatment and SDS-Page was used. The band size is 400 kDa. Flow cytometry - This antibody stains the intra and extracellular domain of Pentraxin 3. For intracellular staining cells were permeabilized with buffer containing triton. Transfected cells were used as a positive control.

Target Information

Pentraxins are a protein superfamily that is characterized by a cyclic multimeric structure. Ptx3, also known as tumor necrosis factor-stimulated gene sequence-14 (TSG14), is a secreted pattern-recognition receptor that has a non-redundant role in resistance to selected microbial agents. Ptx3 belongs to the family of long pentraxins, which have C-terminal pentraxin domains and novel amino-terminal domains. Ptx3 binds selected pathogens, including Aspergillus fumigatus, Pseudomonas aeruginosa and Salmonella typhimurium. It is synthesized in IgA glomerulonephritis and activates mesangial cells. Secretion of Ptx3 in adipose cells can be induced by TNF. Ptx3 is also involved in amplification of inflammatory reactions and regulation of innate immunity. The human PTX3 gene maps to chromosome 3q25.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.