Thermo Fisher Scientific Phospho-JunD (Ser255) Recombinant Rabbit Monoclonal Antibody (JJ08-21)
Phospho-JunD (Ser255) 인산화 단백질을 검출하는 재조합 토끼 단일클론 항체. Western blot, IHC, ICC/IF에 사용 가능. 높은 특이성과 재현성을 제공하며, 동물 유래 성분이 없는 포뮬레이션. TBS 기반 완충액에 보관 용이.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JJ08-21 |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser255 of Human JunD (aa 251–270) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809722 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broad immunoreactivity due to the large rabbit immune repertoire
Target Information
JunD is a transcription factor encoded by the JunD gene. Its transcriptional activity is modulated by phosphorylation in response to cellular stress via the c-Jun N-terminal kinase (JNK)/Stress-Activated Protein Kinase (SAPK) pathway.
Jun proteins can form dimers with Fos, ATF, and CREB family transcription factors to create the AP-1 complex, which regulates genes involved in growth, proliferation, differentiation, and apoptosis.
JunD also plays a role in metabolism through modulation of the IGF-1 signaling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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