Thermo Fisher Scientific REST Recombinant Rabbit Monoclonal Antibody (JE32-25)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

-

Immunohistochemistry (Paraffin) (IHC (P))

1:400

-

Immunocytochemistry (ICC/IF)

1:200

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JE32-25

Immunogen

Recombinant protein within Human REST aa 850-1000 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:REST,uniProtId:Q13127-1,ncbiNodeId:9606,antigenRange:850-1000,antigenLength:1097,antigenImageFileName:MA5-44189_REST_Q13127-1_Rabbit.svg,antigenImageFileNamePDP:MA5-44189_REST_Q13127-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS with 40% glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2926319

Product Specific Information

Predicted band size: 122 kDa

Positive Control: HL-60 cell lysate, HUVEC cell lysate, human thyroid tissue, human seminoma tissue, SH-SY5Y.

Target Information

Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.