Thermo Fisher Scientific TGF-alpha (Transforming Growth Factor alpha) Monoclonal Antibody (MF9)

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

2-4 µg/mL

-

Immunocytochemistry (ICC/IF)

1-2 µg/mL

-

ELISA (ELISA)

2-4 µg/mL

-

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

MF9

Immunogen

A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGFalpha.. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TGF alpha,uniProtId:P01134-1,ncbiNodeId:10114,antigenRange:142-159,antigenLength:159,antigenImageFileName:7039-MSM1-P0_TGF_alpha_P01134-1_House_mouse.svg,antigenImageFileNamePDP:7039-MSM1-P0_TGF_alpha_P01134-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

200 µg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

TGF-alpha is an EGF-related polypeptide growth factor that signals through the EGF receptor, and stimulates the proliferation of a wide range of epidermal and epithelial cells. It is produced by monocytes, keratinocytes, and various tumor cells. TGF-alpha induces anchorage-independence transformation in cultured cells. Human, murine and rat TGF-alpha are cross-species reactive. TGFalpha (aa 50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.