Thermo Fisher Scientific c-MAF Monoclonal Antibody (sym0F1), eFluor 660, eBioscience
Human 및 Mouse c-Maf 단백질을 표적하는 sym0F1 단클론 항체로, eFluor 660 형광 표지. 세포내 염색 및 유세포분석(Flow Cytometry)에 적합. IL-4, IL-21 발현 연구 및 Th17/Tfh 세포 기능 분석에 활용 가능. 4°C 암조건 보관, 동결 금지.
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Thermo Fisher Scientific c-MAF Monoclonal Antibody (sym0F1), eFluor 660, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.125 µg/test
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Fungi, Human, Mouse |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), eFluor™ 660, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | sym0F1 |
| Immunogen | Full-length E. coli-produced c-Maf (Target Family: c-MAF, UniProt ID: O75444-2, Antigen Range: 1–373, Antigen Length: 373) |
| Conjugate | eFluor™ 660 |
| Excitation/Emission Max | 651 / 668 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574387 |
Additional Formats
- Alexa Fluor 488
- Brilliant Violet 421
- Brilliant Violet 480
- eFluor 450
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The sym0F1 monoclonal antibody reacts with human and mouse c-Maf, a 42 kDa basic leucine zipper transcription factor involved in neural, ocular, and hematopoietic systems.
In hematopoietic cells, c-Maf regulates IL-4 expression in Th2 cells and is crucial for Th subset differentiation. It also drives IL-21 expression in Th17 and Tfh cells, promoting IL-23R and CXCR5 expression respectively.
- Applications Reported: Intracellular staining followed by flow cytometric analysis
- Applications Tested: Tested on TCR-activated mouse splenocytes using Foxp3/Transcription Factor Buffer Set (≤0.125 µg/test)
Refer to BestProtocols® for detailed staining procedure.
Fluorochrome Information:
eFluor® 660 replaces Alexa Fluor® 647.
- Excitation: 633–647 nm
- Emission: 668 nm
- Laser: Red Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
c-Maf is a transcription factor belonging to the basic leucine zipper (bZIP) family and the cellular counterpart of oncogenic v-maf.
It regulates IL-4 expression in Th2 cells, crystalline genes in lens fiber cells, insulin gene in islets, and p53/L7 genes via Maf recognition element (MARE).
c-Maf is involved in Th2 differentiation, lineage-specific hematopoiesis, and IL-10 gene expression in LPS-activated macrophages.
Chromosomal aberrations involving maf are associated with multiple myeloma. Expressed in myeloma cell lines and resting monocytes/macrophages.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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