Thermo Fisher Scientific c-MAF Monoclonal Antibody (sym0F1), eFluor 660, eBioscience

Thermo Fisher Scientific c-MAF Monoclonal Antibody (sym0F1), eFluor 660, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 0.125 µg/test
  View 7 publications

Product Specifications

Additional Formats

• Alexa Fluor 488
• Brilliant Violet 421
• Brilliant Violet 480
• eFluor 450
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The sym0F1 monoclonal antibody reacts with human and mouse c-Maf, a 42 kDa basic leucine zipper transcription factor involved in neural, ocular, and hematopoietic systems.
In hematopoietic cells, c-Maf regulates IL-4 expression in Th2 cells and is crucial for Th subset differentiation. It also drives IL-21 expression in Th17 and Tfh cells, promoting IL-23R and CXCR5 expression respectively.

• Applications Reported: Intracellular staining followed by flow cytometric analysis
• Applications Tested: Tested on TCR-activated mouse splenocytes using Foxp3/Transcription Factor Buffer Set (≤0.125 µg/test)
  Refer to BestProtocols® for detailed staining procedure.

Fluorochrome Information:
eFluor® 660 replaces Alexa Fluor® 647.

• Excitation: 633–647 nm
• Emission: 668 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

c-Maf is a transcription factor belonging to the basic leucine zipper (bZIP) family and the cellular counterpart of oncogenic v-maf.
It regulates IL-4 expression in Th2 cells, crystalline genes in lens fiber cells, insulin gene in islets, and p53/L7 genes via Maf recognition element (MARE).
c-Maf is involved in Th2 differentiation, lineage-specific hematopoiesis, and IL-10 gene expression in LPS-activated macrophages.
Chromosomal aberrations involving maf are associated with multiple myeloma. Expressed in myeloma cell lines and resting monocytes/macrophages.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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