
Thermo Fisher Scientific EPN2 Monoclonal Antibody (OTI1C5), TrueMAB
EPN2 단백질을 인식하는 Mouse IgG1 단클론 항체로, Western blot, IHC, ICC/IF에 사용 가능. 인간 시료 반응성. 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도에서 사용. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1C5 |
| Immunogen | Full length human recombinant protein of human EPN2 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Epsin2 is involved in the formation of clathrin-coated invaginations and endocytosis.
It binds to EPS15 (via the NPF repeat domain) and to AP-2 and clathrin (via the DPW repeat domain).
Epsin2 is localized in punctate cytoplasmic structures associated with clathrin-coated vesicles, especially near the Golgi complex.
Highest expression is observed in the brain, with lower levels in the lung and liver.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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