Thermo Fisher Scientific EPN2 Monoclonal Antibody (OTI1F1), TrueMAB
인간 EPN2 단백질을 표적으로 하는 Mouse IgG1 단클론 항체로, WB, IHC, ICC/IF에 적합합니다. 동결건조 형태로 제공되며, 재구성 후 1 mg/mL 농도로 사용 가능합니다. 세포 내 클라트린 매개 엔도사이토시스 연구에 활용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1F1 |
| Immunogen | Full length human recombinant protein of human EPN2 produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Epsin2 plays a role in the formation of clathrin-coated invaginations and endocytosis. It binds to EPS15 (via the NPF repeat domain), AP-2, and clathrin (via the DPW repeat domain). The protein resides in the cytoplasm, associated with clathrin-coated vesicles, and is concentrated near the Golgi complex. Expression is highest in brain, with lower levels in lung and liver.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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