Thermo Fisher Scientific IDO Monoclonal Antibody (eyedio), FITC, eBioscience

Thermo Fisher Scientific IDO Monoclonal Antibody (eyedio), FITC, eBioscience™

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.06 µg)/test
• Publications: View 7 publications

Product Specifications

Available Conjugate Formats

• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 395
• Brilliant UV 615
• Brilliant UV 737
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 786
• eFluor 450
• eFluor 660
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

This eyedio monoclonal antibody reacts with human indoleamine-2,3-dioxygenase (IDO, INDO, IDO1), an intracellular enzyme that catalyzes the degradation of tryptophan to kynurenines. IDO is expressed in various tissues and cells such as macrophages, plasmacytoid dendritic cells, and several cell lines. It can be induced by IFN-γ or other inflammatory stimuli. Expression of IDO in antigen-presenting cells and tumors is thought to mediate immune suppression via tryptophan depletion or production of metabolites that induce T-cell apoptosis and regulatory T-cell formation.

Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Pre-titrated and tested using the Intracellular Fixation & Permeabilization Buffer Set (Product #88-8824-00) following BestProtocols® guidelines.
Use 5 µL (0.06 µg) per test (defined as the amount to stain cells in 100 µL final volume). Recommended cell number: 10⁵–10⁸ cells/test.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IDO1 is an intracellular heme-containing enzyme that catalyzes the oxidative cleavage of the indole ring of tryptophan, serotonin, and melatonin, producing biologically active kynurenines. It is expressed in various human tissues, macrophages, and dendritic cells. IDO1 induction by interferons contributes to antimicrobial responses and immunoregulation in infection, pregnancy, autoimmunity, transplantation, and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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