Thermo Fisher Scientific IDO Monoclonal Antibody (mIDO-48), FITC, eBioscience
마우스 IDO1 단백질을 특이적으로 인식하는 mIDO-48 단클론 항체로, FITC 형광 표지되어 있음. Flow cytometry 분석에 최적화된 시약이며, 488 nm에서 여기되어 520 nm에서 방출. 세포 내 단백질 염색에 적합하며 4°C 암소 보관 권장.
- 카탈로그번호
- 11-9473-82
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IDO Monoclonal Antibody (mIDO-48), FITC, eBioscience
Thermo Fisher Scientific IDO Monoclonal Antibody (mIDO-48), FITC, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 1.0 µg/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | mIDO-48 |
| Conjugate | FITC |
| Excitation/Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2688147 |
Product Specific Information
The monoclonal antibody mIDO-48 reacts with mouse indoleamine 2,3-dioxygenase 1 (IDO1).
IDO1 is an intracellular enzyme expressed by dendritic cells, interferon gamma-activated macrophages, epithelial cells, vascular endothelium, and tumor cells. It catalyzes the conversion of tryptophan into kynurenine metabolites, which suppress T cell proliferation and promote regulatory T cell activation.
Applications Reported:
This antibody is reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00).
Recommended usage ≤ 1.0 µg per test (100 µL final volume, 10⁵–10⁸ cells/test).
Careful titration is advised for optimal performance.
Fluorescence Characteristics:
- Excitation: 488 nm
- Emission: 520 nm
- Laser: Blue Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
IDO1 is an intracellular heme-containing enzyme that catalyzes oxidative cleavage of the indole ring of tryptophan, serotonin, and melatonin, producing biologically active kynurenines.
IDO1 is expressed in various tissues and immune cells such as macrophages and dendritic cells.
Induced by interferons, IDO1 contributes to antimicrobial defense mechanisms and plays a crucial role in immunoregulation during infection, pregnancy, autoimmunity, transplantation, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.