Thermo Fisher Scientific IDO Monoclonal Antibody (mIDO-48), FITC, eBioscience

Thermo Fisher Scientific IDO Monoclonal Antibody (mIDO-48), FITC, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 1.0 µg/test

Product Specifications

Product Specific Information

The monoclonal antibody mIDO-48 reacts with mouse indoleamine 2,3-dioxygenase 1 (IDO1).
IDO1 is an intracellular enzyme expressed by dendritic cells, interferon gamma-activated macrophages, epithelial cells, vascular endothelium, and tumor cells. It catalyzes the conversion of tryptophan into kynurenine metabolites, which suppress T cell proliferation and promote regulatory T cell activation.

Applications Reported:
This antibody is reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00).
Recommended usage ≤ 1.0 µg per test (100 µL final volume, 10⁵–10⁸ cells/test).
Careful titration is advised for optimal performance.

Fluorescence Characteristics:

• Excitation: 488 nm
• Emission: 520 nm
• Laser: Blue Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

IDO1 is an intracellular heme-containing enzyme that catalyzes oxidative cleavage of the indole ring of tryptophan, serotonin, and melatonin, producing biologically active kynurenines.
IDO1 is expressed in various tissues and immune cells such as macrophages and dendritic cells.
Induced by interferons, IDO1 contributes to antimicrobial defense mechanisms and plays a crucial role in immunoregulation during infection, pregnancy, autoimmunity, transplantation, and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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