Thermo Fisher Scientific CD45 Monoclonal Antibody (2D1), FITC, eBioscience

Thermo Fisher Scientific CD45 Monoclonal Antibody (2D1), FITC, eBioscience

Applications and Tested Dilution

• Immunohistochemistry (Paraffin) (IHC (P)): 1:100
• Flow Cytometry (Flow): 5 µL (1 µg)/test

Product Specifications

Available Formats

• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant Violet 711
• eFluor 450
• NovaFluor Blue / Red / Violet / Yellow series
• PE, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436 / 600 / 645 / 780
• Custom conjugation available

Product Specific Information

The 2D1 monoclonal antibody reacts with all isoforms of human CD45 (Leukocyte Common Antigen, LCA). CD45 is expressed by all hematopoietic cells except circulating erythrocytes and platelets. Its cytoplasmic domain has tyrosine phosphatase activity, playing a key role in lymphocyte activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated for flow cytometry using human peripheral blood leukocytes
• Suggested Use: 5 µL (1.0 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)
• Excitation: 488 nm
• Emission: 520 nm
• Laser: Blue Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD45 (LCA) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells, comprising ~10% of lymphocyte surface proteins. Absent in non-hematopoietic tissues, CD45 regulates Src-family kinases, T/B-cell receptor signaling, and cytokine receptor pathways. Isoforms arise via alternative splicing and differ by extracellular domain composition. CD45 is critical for lymphocyte development, activation, and survival.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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