Thermo Fisher Scientific CCDC134 Recombinant Rabbit Monoclonal Antibody (026)

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Immunoprecipitation (IP)

• Tested Dilution: 1–4 µL/mg of lysate

Product Specifications

Product Specific Information

This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal clones for production.
Advantages include:

• Better specificity and sensitivity
• Lot-to-lot consistency
• Animal origin–free formulations
• Broader immunoreactivity due to larger rabbit immune repertoire

This antibody has specificity for Human CCDC134.

Target Information

The coiled-coil domain is a common protein motif involved in protein oligomerization, found in transcription factors and intermediate filaments.
CCDC134 is a secretory protein that inhibits the transcriptional activity of Elk1. Overexpression of CCDC134 inhibits phosphorylation of Erk and JNK/SAPK but not p38 MAPK, while siRNA against CCDC134 activates Elk1 transcriptional activity and phosphorylation of Erk and JNK/SAPK.
This suggests a potential inhibitory role of CCDC134 in MAPK-mediated Elk1 transcription.
CCDC134 is widely expressed in normal adult tissues, tumors, and cell lines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지

(이미지 없음)