Thermo Fisher Scientific AF9 Polyclonal Antibody
Rabbit 폴리클로날 항체로 AF9 단백질(MLLT3)을 인식하며 Western blot, IHC, IP 등에 적합. 비결합형 액상 형태로 제공되며 4°C 보관. FFPE 조직에서 citrate buffer(pH 6.0)로 항원 회복 권장.
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Thermo Fisher Scientific AF9 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:15,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 1–4 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residues 250–300 of human ALL1 Fused Gene from Chromosome 9 Protein (MLLT3, Myeloid/Lymphoid or Mixed-Lineage Leukemia Translocate 3) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
The human AF9 gene is one of the most common fusion partner genes with the ALL1 gene at 11q23 (also called MLL), resulting in the t(9;11)(p22;q23) translocation.
AF9 spans more than 100 kb and contains two patient breakpoint cluster regions (BCRs):
- BCR1: within intron 4 (site A)
- BCR2: spans introns 7 and 8 (site B)
Structural elements identified in AF9 include:
- DNA topo II cleavage sites and DNase I hypersensitive sites in intron 7 (BCR2)
- Two scaffold-associated regions (SARs): SAR1 in intron 4 and SAR2 encompassing exons 5–7
These regions share structural similarities with the MLL breakpoint cluster region, supporting a DNA breakage and repair model for nonhomologous recombination between MLL and AF9.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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