Thermo Fisher Scientific AF9 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000–1:10,000

Immunoprecipitation (IP)

• Tested Dilution: 2–10 µg/mg lysate

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt.

Target Information

The human AF9 gene is one of the most common fusion partner genes with the ALL1 (MLL) gene at 11q23, resulting in the t(9;11)(p22;q23) translocation.
The AF9 gene spans over 100 kb and contains two patient breakpoint cluster regions (BCRs):

• BCR1: within intron 4 (previously called site A)
• BCR2: spans introns 7 and 8

Several structural elements have been identified in AF9, including:

• A colocalizing in vivo DNA topo II cleavage site
• An in vitro DNase I hypersensitive site in intron 7 (BCR2)
• Two scaffold-associated regions (SARs): SAR1 (intron 4) and SAR2 (exons 5–7)

These breakpoint regions share structural similarity with the MLL BCR, suggesting a DNA breakage and repair model for nonhomologous recombination between MLL and AF9.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.