Thermo Fisher Scientific Phospho-EGFR (Tyr1068) Recombinant Rabbit Monoclonal Antibody (2H9L3)

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:5,000
• View 1 publication

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:100–1:1,000

Product Specifications

Product Specific Information

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generates a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.

Advantages of recombinant rabbit monoclonal antibodies:

• Better specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulations
• Broader immunoreactivity due to larger rabbit immune repertoire

Target Information

EGFR (epidermal growth factor receptor) is a receptor tyrosine kinase that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer, and EGFR is the target of both biological and small molecular therapeutics.

EGFR is encoded by the EGFR gene located on chromosome 7 in humans and belongs to the HER/ErbB family of proteins, which includes ERbB2, ERbB3, and ERbB4.

EGFR is a transmembrane receptor; binding of ligands such as EGF and TGF-α to the extracellular domain leads to dimerization and autophosphorylation of tyrosine residues in the cytoplasmic domain.

Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, and squamous cell carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.