Thermo Fisher Scientific JNK1 Recombinant Rabbit Monoclonal Antibody (2H15L8)
Recombinant rabbit monoclonal antibody specific for JNK1 (MAPK8). Validated for WB and ICC/IF applications. High specificity and lot-to-lot consistency with 0.5 mg/mL concentration. Suitable for human samples; predicted cross-reactivity with mouse.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–3 µg/mL |
| Immunocytochemistry (ICC/IF) | 1 µg/mL |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 2H15L8 |
| Immunogen | Recombinant protein corresponding to amino acids 17–126 of human c-Jun N-terminal kinase 1 (JNK1, UniProt ID: P45983-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2532355 |
Product Specific Information
- Predicted to react with mouse based on sequence homology.
- On a non-reducing gel, intact IgG appears as a ~150 kDa band; upon reduction, generates ~25 kDa light chain and ~50 kDa heavy chain bands.
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems, developed by cloning specific antibody DNA sequences from immunoreactive rabbits.
- Advantages include:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
MAPK8 (JNK1) is activated by misfolded proteins in the endoplasmic reticulum and is a crucial mediator of insulin resistance and obesity. It belongs to the MAPK superfamily of stress-activated protein kinases. MAPKs are serine-threonine protein kinases activated by various extracellular stimuli, mediating signal transduction from the cell surface to the nucleus.
JNK participates in cellular processes such as proliferation, differentiation, transcription regulation, and development. JNK pathways are activated by stress and inflammatory signals. JNK exists as ten different isoforms due to differential mRNA splicing, with JNK1 and JNK2 being predominant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일명: 701048_JNK1_P45983-1_Rabbit.svg, 701048_JNK1_P45983-1_Rabbit_PDP.jpeg)
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