Thermo Fisher Scientific MMP9 (Matrix Metalloproteinase 9) Recombinant Rabbit Monoclonal Antibody (MMP9, 2025R)

Applications

• Immunohistochemistry (Paraffin) (IHC (P)): 1–2 µg/mL

Product Specifications

Product Specific Information

• For IHC (PFA fixed): Incubate antibody for 30 minutes at room temperature.
• Staining of formalin-fixed tissues requires heating sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

MMP9 (matrix metallopeptidase 9, also known as GELB or CLG4B) is a zinc- and calcium-dependent endopeptidase that degrades extracellular matrix proteins. It is secreted as a 92 kDa zymogen, with the active enzyme at 82 kDa after cleavage. MMP9 contains:

• A gelatin-binding domain (three fibronectin type II units)
• A catalytic domain with a zinc-binding site
• A proline-rich type V collagen-homologous domain
• A hemopexin-like domain

MMP9 is expressed in monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts, and endothelial cells. It plays roles in:

• Inflammatory responses
• Tissue remodeling and wound healing
• Tumor growth and metastasis

MMP9 contributes to skin carcinogenesis and degrades type IV and V collagens. Studies indicate involvement in IL-8-induced mobilization of hematopoietic progenitor cells and tumor-associated tissue remodeling. Elevated MMP9 levels are associated with idiopathic atrial fibrillation and aortic aneurysm progression.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.