Thermo Fisher Scientific Human GUSB (Beta Glucuronidase) Endogenous Control (VIC/MGB probe, primer limited)

Applied Biosystems™ Human GUSB (Beta Glucuronidase) Endogenous Control (VIC™/MGB Probe, Primer Limited)

The Applied Biosystems™ Human GUSB (beta glucuronidase) Endogenous Control (VIC™/MGB Probe, Primer Limited) is intended as an endogenous control for relative gene expression quantification in cDNA samples when used with other gene expression assays.
Probe is labeled with VIC™ dye-MGB, and the primers are limited. Can be used for multiplex or singleplex PCR reactions.
Compatible with Inventoried and Non-Inventoried TaqMan™ Gene Expression Assays, Custom TaqMan™ Gene Expression Assays, and/or Custom TaqMan™ Primers and Probes.

Assay Details

TaqMan™ Endogenous Controls

TaqMan™ Endogenous Controls eliminate months of assay design, formulation, and testing by providing pre-designed probe and primer sets to normalize RNA or DNA amounts in reactions.

Complete Solution for Quantitative Gene Expression

TaqMan™ Endogenous Controls simplify the selection of appropriate controls for human, mouse, rat, and eukaryotic genes, reducing trial-and-error in assay setup.

Simple to Use

All components are QC tested and formulated into a single 20X mix. Compatible with universal conditions for two-step RT-PCR.
Use with TaqMan™ Universal PCR Master Mix (with or without AmpErase™ UNG) and cDNA samples on Applied Biosystems instruments (7900HT, 7300, 7500, 7000, 7700).

Flexible Offering

Choose between different reporter dyes and quenchers:

• FAM™ dye-labeled TaqMan™ MGB probe (250 nM) with two unlabeled PCR primers (900 nM each)
• VIC™ dye-labeled TaqMan™ MGB probe (250 nM) with two unlabeled PCR primers (150 nM each, primer limited)
• VIC™ dye-labeled TAMRA™ probe (250 nM) with two unlabeled PCR primers (150 nM each, primer limited)

Choosing the Right Endogenous Control

Endogenous controls normalize target gene expression by correcting cDNA input differences.
Ensure consistent expression across all samples and verify that the endogenous control gene is more abundantly expressed than the target for multiplexing.

Multiplex vs. Singleplex PCR

• VIC™ reporter dye-labeled controls are primer limited and suitable for multiplexing.
• FAM™ reporter dye-labeled controls are not primer limited and are intended for singleplex assays.

For Research Use Only. Not for use in diagnostic procedures.

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