Thermo Fisher Scientific SSEA4 Polyclonal Antibody
인간 SSEA-4 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC/IF에 사용 가능. 줄기세포 표지자 연구에 적합하며, 항원 친화 크로마토그래피로 정제됨. 액상 형태로 4°C 단기 보관, -20°C 장기 보관 권장.
- 카탈로그번호
- 620-500
- 판매단위
- pk
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Thermo Fisher Scientific SSEA4 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
| Immunocytochemistry (ICC/IF) | 1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide derived from the C-terminus of human SSEA-4 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: TRA1
- Cellular location: Membrane, Cytoplasm
Target Information
SSEA-4 (Stage-specific embryonic antigen 4) is a cell surface antigen expressed along with SSEA-3, TRA-1-60, and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells, and induced pluripotent stem cells (iPS).
In human cells, these surface markers are down-regulated during differentiation, while SSEA-1 is absent in undifferentiated human stem cells and upregulated after retinoic acid-mediated differentiation.
In mouse stem cells, SSEA-1 expression decreases with differentiation, while SSEA-3 and SSEA-4 expression increase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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