Thermo Fisher Scientific CD47 Monoclonal Antibody (2D3), Super Bright 436, eBioscience
CD47 단백질을 인식하는 2D3 클론 단일클론 항체로, 인간 시료에 반응합니다. Super Bright 436 형광체로 결합되어 유세포분석(Flow Cytometry)에 최적화되어 있습니다. 고감도 검출 및 비특이적 결합 최소화를 위해 Super Bright Staining Buffer 사용을 권장합니다.
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Thermo Fisher Scientific CD47 Monoclonal Antibody (2D3), Super Bright 436, eBioscience™
Applications
- Flow Cytometry (Flow)
Tested dilution: 5 µL (1.0 µg)/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright™ 436 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2D3 |
| Conjugate | Super Bright™ 436 |
| Excitation / Emission Max | 413 / 431 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2762514 |
Additional Formats
- Unconjugated
- FITC
- NovaFluor Blue 510
- NovaFluor Blue 585
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Blue 690
- NovaFluor Red 710
- NovaFluor Red 725
- NovaFluor Yellow 570
- NovaFluor Yellow 660
- NovaFluor Yellow 730
- NovaFluor Yellow 755
- Request custom conjugation available
Product Specific Information
The monoclonal antibody 2D3 reacts with CD47, also known as integrin-associated protein (IAP) and neurophilin.
CD47 is a glycosylated five-transmembrane protein involved in adhesion through interactions with SIRP (signal regulator protein) and integrins (CD51/CD61, CD41/CD61).
It mediates bi-directional signaling, influences neural synaptic activity, and regulates macrophage phagocytosis.
CD47 acts as a receptor for thrombospondin and is expressed in most hematopoietic cells (T cells, B cells, monocytes, platelets, erythrocytes) and various non-hematopoietic cells.
Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Pre-titrated and validated for flow cytometry using normal human peripheral blood cells at 5 µL (1 µg) per test.
A test is defined as staining a cell sample in 100 µL final volume (cell number: 10⁵–10⁸ cells/test).
Fluorochrome Characteristics:
- Excitation: 405 nm
- Emission: 436 nm
- Recommended filter: 450/50 bandpass
Ensure instrument compatibility for Super Bright 436 detection.
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD47 (Integrin-associated protein, IAP) is a ubiquitously expressed transmembrane glycoprotein interacting with integrins and regulating their functions.
It modulates signaling pathways involving heterotrimeric G proteins and intracellular calcium increase upon cell adhesion.
CD47 serves as a receptor for thrombospondin and plays roles in membrane transport and signal transduction.
It prevents phagocytosis of CD47-positive cells via interaction with CD172a (SIRPα) and influences cell migration, B cell adhesion, T cell activation, and neuronal development.
For Research Use Only. Not for diagnostic procedures or resale without authorization.
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