Thermo Fisher Scientific CD47 Monoclonal Antibody (2D3), Super Bright 436, eBioscience

Thermo Fisher Scientific CD47 Monoclonal Antibody (2D3), Super Bright 436, eBioscience™

Applications

• Flow Cytometry (Flow)
  Tested dilution: 5 µL (1.0 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 585
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Yellow 570
• NovaFluor Yellow 660
• NovaFluor Yellow 730
• NovaFluor Yellow 755
• Request custom conjugation available

Product Specific Information

The monoclonal antibody 2D3 reacts with CD47, also known as integrin-associated protein (IAP) and neurophilin.
CD47 is a glycosylated five-transmembrane protein involved in adhesion through interactions with SIRP (signal regulator protein) and integrins (CD51/CD61, CD41/CD61).
It mediates bi-directional signaling, influences neural synaptic activity, and regulates macrophage phagocytosis.
CD47 acts as a receptor for thrombospondin and is expressed in most hematopoietic cells (T cells, B cells, monocytes, platelets, erythrocytes) and various non-hematopoietic cells.

Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Pre-titrated and validated for flow cytometry using normal human peripheral blood cells at 5 µL (1 µg) per test.
A test is defined as staining a cell sample in 100 µL final volume (cell number: 10⁵–10⁸ cells/test).

Fluorochrome Characteristics:

• Excitation: 405 nm
• Emission: 436 nm
• Recommended filter: 450/50 bandpass
  Ensure instrument compatibility for Super Bright 436 detection.

When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD47 (Integrin-associated protein, IAP) is a ubiquitously expressed transmembrane glycoprotein interacting with integrins and regulating their functions.
It modulates signaling pathways involving heterotrimeric G proteins and intracellular calcium increase upon cell adhesion.
CD47 serves as a receptor for thrombospondin and plays roles in membrane transport and signal transduction.
It prevents phagocytosis of CD47-positive cells via interaction with CD172a (SIRPα) and influences cell migration, B cell adhesion, T cell activation, and neuronal development.

For Research Use Only. Not for diagnostic procedures or resale without authorization.

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