Thermo Fisher Scientific ID3 Monoclonal Antibody (OTI5C7)
ID3 단백질을 인식하는 Thermo Fisher Scientific의 마우스 단클론 항체로, Western blot, IHC, ICC/IF, Flow cytometry에 사용 가능. 1 mg/mL 농도의 액상 형태로 제공되며, PBS 버퍼에 1% BSA와 50% 글리세롤이 포함됨. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5C7 |
| Immunogen | Full length human recombinant protein of ID3 produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2724496 |
Target Information
Members of the Id family of basic helix-loop-helix (bHLH) proteins include ID1, ID2, ID3, and ID4. They are ubiquitously expressed and dimerize with members of the class A and B HLH proteins. Due to the absence of the basic region, the resulting heterodimers cannot bind DNA, leading to negative regulation of DNA binding by bHLH proteins.
ID1 inhibits DNA binding of E12 and Myo D, functioning to suppress muscle-specific gene expression. Under differentiation conditions, ID protein levels decrease, allowing E12/E47 to dimerize with Myo D and myogenin, thereby activating myogenic differentiation. Expression of ID proteins is strongly dependent on growth factor activation, and reduction of ID mRNA levels delays cell cycle reentry after growth factor stimulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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